H2O and HDO differ profoundly in their infrared optical properties. A strong absorbance band in the 1650-1750 cm-1 region indicate that a carbonyl group (CO) is. It is a very strong band comparing to the others on the spectrum. Also, when you read, make sure you keep track of the isotopic mixture being discussed. For example, the most characteristics absorption band in the spectrum of 2-hexanone ( Figure 6.3a) is that from the stretching vibration of carbonyl double bond CO, at 1716 cm -1. A, Transmission absorbance spectra of water (55M), hemoglobin (approximately 100 mg/mL), concanavalin (approximately 20 mg/mL), and lysozyme (approximately 100 mg/mL) in transmission mode with a cell with 4 m spacer but cell assembled to 710 m path length. What the plot shows is the reciprocal path length needed for a given absorbance to apply, for example slightly less than 100 m in the visible to $10^$ water vibrational spectrum.
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